LOCUS       V004389                10047 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004389
VERSION     V004389
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Inui M, Tsuge Y, Suzuki N, Vertes AA, Yukawa H.
  TITLE     Isolation and characterization of a native composite transposon,
            Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum
            chromosomal DNA
  JOURNAL   Appl. Environ. Microbiol. 71 (1), 407-416 (2005)
   PUBMED   15640215
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Inui M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2004) Masayuki Inui, Research Institute of
            Innovative Technology for the Earth (RITE), Microbiology Research
            Group; 9-2 Kizugawadai, Kizu, Soraku, Kyoto 619-0292, Japan
            (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, Fax:81-774-75-2321)
REFERENCE   3  (bases 1 to 10047)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10047)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2005"; volume: "71"; issue: "1"; pages:
            "407-416"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2004) Masayuki Inui, Research Institute of Innovative
            Technology for the Earth (RITE), Microbiology Research Group";
            volume: " 9-2 Kizugawadai, Kizu, Soraku, Kyoto 619-0292, Japan
            (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, Fax"; pages:
            "81-774-75-2321"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             870..2357
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label="repA"
                     /protein_id="BAD83816.1"
                     /translation="MYMIIPNSTPAYPISGSRVNTPSRLRPDCAGLDDTPASTVDRDKL
                     LAHLGRTALHGSISRNFKGAYRLVVDKETGEKRSVPNLYRIDSEKLGRCEYVMLTSKQY
                     ASVMVIDVDQIGEAGGHPENLNSYVKGVIWVLVQHGIGPAWAGINPISGKAQFIWLIDP
                     VYAGKNRASRNMELLKATSHELGELLDHDPHFAHRFSRSPFYTGKSPEAYRWYCQHDRV
                     IRLQDFLRQVREMAGQSQHIKNKRQQFNSGRELINAVKTRREEAQAFKALAEDVENEIS
                     EEIDQYDPELIDGVRVRWISQGVAARDETAFSHALKIGHRLRKAGQRLTDAAVIDAYEH
                     AYNVAQQQGSAGRDSEMPPMRDRQTMARRVRGYVTQSKTNTSLGASAPGRVTSSERKAL
                     ATMGRKGGQKAAQRWKTDPEGQYAQNQLQKLKKTHRKKRVEGQTTRAKIQALIGEAYVQ
                     TGEVLTRKQIVDETGLSRATVTRHLAALREQGALPET"
     gene            870..2357
                     /gene="repA"
                     /label="repA"
     regulatory      2067..2076
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4213..4229)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    4237..4253
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4261..4291)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4306..4327)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4615..5203)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     5692..5708
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5749..6194
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
     CDS             6195..7613
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     CDS             7823..8602
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     mobile_element  complement(8764..9531)
                     /label="IS1"
                     /note="prokaryotic transposable element"
     primer_bind     10005..10021
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ggtatttgaa tgataaacag aaaaaagcca ggaagagtac ttttacgaaa agcagtccat
       61 ttattttgac tccggcattc caatcgcgat aggtgacaac acaccatttg aatccgtaaa
      121 ggatgaagag cactataaaa aatgctatcg agcaagctct atctacagct gttgcttccg
      181 gggacgtcag actcagagct gccattgaaa aaattacggt agaaaacaga ggcatgcatg
      241 tctcgtggat gactaatttc cacttagcgg gtttgctcag tcttttaagg aactgctcag
      301 cggtatcttt gtcctggaat gttgcttcat gattgaccgt agtggagtct tggcacttag
      361 caccgtcgcc aggaacattc ggtggattcc tctcgctagt gataatgcgg ataagtccca
      421 atagcaggat gaaactgctg atgattaagg cttgtgtggg ggcggtagat tttttggtct
      481 tagaaaagaa agaagtcata cggcacatca tgcccaccgg tagaaagccc gtgctcaact
      541 attaacgcca tggaaaccac tcagttttta agcaatgtat aactcttcta cccgcatgaa
      601 taagcgagat acccatcgct ggatctcttc ccctacccct ggtagatatt gcgcagcctg
      661 accatggaca tgcttctacg ttcttccaca ctggtatctt ttggttgcaa tatatattgc
      721 aaccatgctg gtaaaacaac tttttagcgt gtcttgccca aaattacagt catgtgattt
      781 acagttgaaa gatactgaaa atcaaagaag gccactcggc ggcaaccgaa tgaccttcgc
      841 ttatcaccca gtcctacctg ggagaaagca tgtacatgat tatacccaat agtacgcccg
      901 cgtaccccat ttccggttcg cgtgttaaca cgccatcacg tttgcgccct gactgcgcag
      961 gccttgatga caccccagcg agcaccgttg accgggacaa gctgctcgca catcttggcc
     1021 gtacagcact gcacgggtct ataagtcgaa acttcaaagg cgcttatcgc ctcgttgtgg
     1081 ataaggaaac gggggaaaag cgaagcgttc cgaaccttta ccgcattgat tccgaaaaac
     1141 tcggtcgctg cgaatacgtc atgctgacta gtaagcaata tgcttcggtc atggtcatag
     1201 acgttgacca gataggagag gctggaggac atccagaaaa cctcaactcc tatgtcaaag
     1261 gcgttatctg ggtacttgtg cagcacggaa ttggaccagc atgggcaggc attaatccga
     1321 ttagtggtaa agcgcagttt atttggctta ttgacccagt ttatgcagga aagaatcgtg
     1381 cgtcccggaa tatggagcta ctcaaagcca caagtcacga gttgggtgag ttactggatc
     1441 atgatccaca ttttgcgcat cggtttagcc ggagtccttt ttatactgga aagtcaccgg
     1501 aggcttatcg ctggtattgc cagcatgacc gggttatacg cctccaagac tttctaaggc
     1561 aggtgcgcga gatggcggga caatcccagc acattaaaaa caagcgccag caatttaaca
     1621 gtggccgtga gcttattaat gcggtcaaaa ctcgccgtga agaagcccaa gcctttaaag
     1681 cacttgctga ggatgtcgaa aacgagataa gtgaagaaat cgatcaatac gacccggaac
     1741 taatcgacgg ggtgcgcgtg cgctggatta gccaaggggt cgcagcacgc gacgaaacag
     1801 cgtttagcca cgcactaaaa attggtcacc gcctacgcaa agcaggacaa cgactcacag
     1861 acgccgccgt tatcgatgcc tacgagcatg cctataacgt tgcacaacaa caaggctcag
     1921 caggccgaga cagcgaaatg ccacccatgc gtgaccgcca gaccatggca cgacgcgtac
     1981 gcggctatgt cacccaatcc aaaaccaaca caagtctagg agctagcgct cccggacgcg
     2041 ttaccagcag cgaacgcaaa gcactggcca ccatggggcg aaaaggcgga cagaaagcag
     2101 cacaacgctg gaaaaccgat ccagaaggtc aatacgcaca aaatcagctg cagaaactaa
     2161 agaaaacgca ccggaagaag cgggtggaag gacagaccac gcgtgcgaag attcaagcct
     2221 taattggtga agcttacgtg caaacaggcg aggtacttac ccgcaaacag attgtggatg
     2281 agacaggact atctagagct acagtgacac ggcatttggc ggcacttcga gaacagggag
     2341 cacttccgga aacgtagggg ctcataccgt aagcaatata cggttcccct gccggtaggc
     2401 agttagtaat aacctctctt gaagaaaacc ttgtagggca aggctactta tgcttccggg
     2461 gttagtcgtt cttctattgc ggtgatgagt tctagacctt tatctaagtc ctggggctgc
     2521 tgttgccgtg cgaggctttg ctgcgcggcg tgaatgattg agcgtagctg tcgcatgttg
     2581 gggaatagtg gggaagtggg tgagaggagt ctggctagtt cggtgagggt gcgtgctgcg
     2641 ttgtctgccg cgtccttggc ttctgcgtag tctctagttc gtgcgtacgc gatgataaat
     2701 acgtagtgta ggtgcgtgtt ttcgattgct tgctgggcgg gtgtagtcat cgttttttag
     2761 agttgaatac ttttgcttcc ctgtttgctt tcaaagcggc taaaacgata atcactattt
     2821 ggatcaaaat agccaagata gggaaaatat agattacgcc aagcgatgag gggctgtcgt
     2881 tgccgaaaag aattggcacg gagatagatg tagcaatccc cactgctccg cagactgtcg
     2941 tggcttgcaa atagggtaga gtttttcgat gccccacttc ccaagcttcg tcagatgcca
     3001 gagtggaacg cattcggaat cccagaactt cgttccgctg taagtttcct gtcttaatcg
     3061 agtgatgcat cccaagacta aggaatgtca aaaggaatat tccaactatt agcgatacgt
     3121 atcctcccac cggtctcctt tccatttaga tctattacgc aagaaaatgc taaacgagct
     3181 ggtaaaattg gtatatttgt ttgacaataa acatatctag gtgcgccatc aatggtgtcc
     3241 ggtggcggac aaggcggttt gtctgagcca cgatcgcgca tgaccgcttt catgggccga
     3301 ttgacagtac cctctagaat ttccccgttc ggagctgaaa ttattgtcta gatactccag
     3361 tattttcaga ccaaacccta gttccaactc ccgccacagc taggagtact aagaaaaccg
     3421 cgagaatgcc ggggcttatc aagttctgcc accacgctac gggacacagc ccaacgtcgt
     3481 cgcagaactc gttggcgtag agcttggcta tgagcgtcat tcttccggac cagccctatc
     3541 gggcaagcca gatcggaatc agctaaccat gcagcaaacc cgactcagtt tatgcgtcct
     3601 tgggtgtcac catttccgcg ggcgtcacta tactaacatg ttagtatagt ttgatcattg
     3661 taatttagag agggaactgg aattaatgag tagtgtgacg tcgtcatctc ggaggtggtg
     3721 gttgcttgtc acggttgggg cagggctgct gctgatcact ctggacaact cgatcctgta
     3781 caccgcgatg ccgaccttga cggaggaact tggcgcgtcg gggtctaccg ctttgtggat
     3841 cattaacgca tacccagtcg tgatggcggg ccttctgctg ggggcgggga ctttgggtga
     3901 ccgcatcgga catcggctga tgttcgttgt aggtcttatt gttttcggct tggcgtcttt
     3961 gacggcggca ttctctgtga gccccgagat gctgattgcc tcgcgtgcat tgctggcggt
     4021 gggtgcggct gcgatgatgc cggcaacgct ggctctgatc cgactcgctt tcgacgatgt
     4081 aaaagaacga aacctagcga tcgcgatttg gggaagcttg tccgtcgtcg gtgcagcgtt
     4141 agggccgatt cttggcggca cactactcga ctctagagga tccccgggta ccgagctcga
     4201 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4261 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4321 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4381 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4441 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4501 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4561 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4621 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4681 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4741 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4801 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4861 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4921 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4981 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5041 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5101 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5161 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5221 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5281 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5341 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5401 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5461 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5521 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccgaggctg cttttctgtt
     5581 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     5641 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     5701 cggccagtga attcgagctc gcccggggat cgatcctttt taacccatca catatacctg
     5761 ccgttcacta ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa
     5821 ggcaacttta tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga
     5881 tagatttttt agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac
     5941 aaaagaggaa aatagaccag ttgcaatcca aacgagagtc taatagaatg aggtcgaaaa
     6001 gtaaatcgcg cgggtttgtt actgataaag caggcaagac ctaaaatgtg taaagggcaa
     6061 agtgtatact ttggcgtcac cccttacata ttttaggtct ttttttattg tgcgtaacta
     6121 acttgccatc ttcaaacagg agggctggaa gaagcagacc gctaacacag tacataaaaa
     6181 aggagacatg aacgatgaac atcaaaaagt ttgcaaaaca agcaacagta ttaaccttta
     6241 ctaccgcact gctggcagga ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc
     6301 catataagga aacatacggc atttcccata ttacacgcca tgatatgctg caaatccctg
     6361 aacagcaaaa aaatgaaaaa tatcaagttc ctgaattcga ttcgtccaca attaaaaata
     6421 tctcttctgc aaaaggcctg gacgtttggg acagctggcc attacaaaac gctgacggca
     6481 ctgtcgcaaa ctatcacggc taccacatcg tctttgcatt agccggagat cctaaaaatg
     6541 cggatgacac atcgatttac atgttctatc aaaaagtcgg cgaaacttct attgacagct
     6601 ggaaaaacgc tggccgcgtc tttaaagaca gcgacaaatt cgatgcaaat gattctatcc
     6661 taaaagacca aacacaagaa tggtcaggtt cagccacatt tacatctgac ggaaaaatcc
     6721 gtttattcta cactgatttc tccggtaaac attacggcaa acaaacactg acaactgcac
     6781 aagttaacgt atcagcatca gacagctctt tgaacatcaa cggtgtagag gattataaat
     6841 caatctttga cggtgacgga aaaacgtatc aaaatgtaca gcagttcatc gatgaaggca
     6901 actacagctc aggcgacaac catacgctga gagatcctca ctacgtagaa gataaaggcc
     6961 acaaatactt agtatttgaa gcaaacactg gaactgaaga tggctaccaa ggcgaagaat
     7021 ctttatttaa caaagcatac tatggcaaaa gcacatcatt cttccgtcaa gaaagtcaaa
     7081 aacttctgca aagcgataaa aaacgcacgg ctgagttagc aaacggcgct ctcggtatga
     7141 ttgagctaaa cgatgattac acactgaaaa aagtgatgaa accgctgatt gcatctaaca
     7201 cagtaacaga tgaaattgaa cgcgcgaacg tctttaaaat gaacggcaaa tggtacctgt
     7261 tcactgactc ccgcggatca aaaatgacga ttgacggcat tacgtctaac gatatttaca
     7321 tgcttggtta tgtttctaat tctttaactg gcccatacaa gccgctgaac aaaactggcc
     7381 ttgtgttaaa aatggatctt gatcctaacg atgtaacctt tacttactca cacttcgctg
     7441 tacctcaagc gaaaggaaac aatgtcgtga ttacaagcta tatgacaaac agaggattct
     7501 acgcagacaa acaatcaacg tttgcgccaa gcttcctgct gaacatcaaa ggcaagaaaa
     7561 catctgttgt caaagacagc atccttgaac aaggacaatt aacagttaac aaataaaaac
     7621 gcaaaagaaa atgccgatac tctagaggat cgatctgtat aataaagaat aattattaat
     7681 ctgtagacaa attgtgaaag gatgtactta aacgctaacg gtcagcttta ttgaacagta
     7741 atttaagtat atgtccaatc tagggtaagt aaattgagta tcaatataaa ctttatatga
     7801 acataatcaa cgaggtgaaa tcatgagcaa tttgattaac ggaaaaatac caaatcaagc
     7861 gattcaaaca ttaaaaatcg taaaagattt atttggaagt tcaatagttg gagtatatct
     7921 atttggttca gcagtaaatg gtggtttacg cattaacagc gatgtagatg ttctagtcgt
     7981 cgtgaatcat agtttacctc aattaactcg aaaaaaacta acagaaagac taatgactat
     8041 atcaggaaag attggaaata cggattctgt tagaccactt gaagttacgg ttataaatag
     8101 gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa tttatatacg gtgagtggct
     8161 caggggtgaa tttgagaatg gacaaattca ggaaccaagc tatgatcctg atttggctat
     8221 tgttttagca caagcaagaa agaatagtat ttctctattt ggtcctgatt cttcaagtat
     8281 acttgtctcc gtacctttga cagatattcg aagagcaatt aaggattctt tgccagaact
     8341 aattgagggg ataaaaggtg atgagcgtaa tgtaatttta accctagctc gaatgtggca
     8401 aacagtgact actggtgaaa ttacctcgaa agatgtcgct gcagaatggg ctatacctct
     8461 tttacctaaa gagcatgtaa ctttactgga tatagctaga aaaggctatc ggggagagtg
     8521 tgatgataag tgggaaggac tatattcaaa ggtgaaagca ctcgttaagt atatgaaaaa
     8581 ttctatagaa acttctctca attaggctaa ttttattgca ataacaggtg cttactttta
     8641 aaactactga tttattgata aatatcctat tggcattttc ttttatttct tatcaacata
     8701 aaggtgaatc ccatatgaac tatataaaag caggcaaatg gctaaccgta ttcctaacct
     8761 tttggtaatg actccaactt attgatagtg ttttatgttc agataatgcc cgatgacttt
     8821 gtcatgcagc tccaccgatt ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg
     8881 ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg
     8941 cagctttccc ttcaggcggg attcatacag cggccagcca tccgtcatcc atatcaccac
     9001 gtcaaagggt gacagcaggc tcataagacg ccccagcgtc gccatagtgc gttcaccgaa
     9061 tacgtgcgca acaaccgtct tccggagact gtcatacgcg taaaacagcc agcgctggcg
     9121 cgatttagcc ccgacatagc cccactgttc gtccatttcc gcgcagacga tgacgtcact
     9181 gcccggctgt atgcgcgagg ttaccgactg cggcctgagt tttttaagtg acgtaaaatc
     9241 gtgttgaggc caacgcccat aatgcgggct gttgcccggc atccaacgcc attcatggcc
     9301 atatcaatga ttttctggtg cgtaccgggt tgagaagcgg tgtaagtgaa ctgcagttgc
     9361 catgttttac ggcagtgaga gcagagatag cgctgatgtc cggcggtgct tttgccgtta
     9421 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
     9481 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ctaacctttt
     9541 taggaatatt gctgtttatc gacttatttc caaaagaaga acatgaccaa aaaacaaaat
     9601 caaaacagaa gccggactac cgggcggcat atcattttac gacgccggat aaatggaaaa
     9661 atgaccctca aaaaccgatc tattttgatg gcaagtatca ttatttctat ctatataacc
     9721 gggattaccc aaaaggcaac ggcacagaat ggcggatccg gggaattgct cgatgagttt
     9781 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     9841 gacggcggct ttgttgaata aatcgcattc gccattcagg ctgcgcaact gttgggaagg
     9901 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     9961 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10021 tgccaagctt gcatgcctgc aggtcga
//
